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(NH4)2SO4
-
NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion
Ca2+
-
maximal activity at 20 mM
Cd2+
inhibits enzymatic activity
Co2+
inhibits enzymatic activity
Cu2+
inhibits enzymatic activity
Fe2+
inhibits enzymatic activity
Fe3+
inhibits enzymatic activity
KCl
-
NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion
MgCl2
-
25 mM for activity assay
Na2SO4
-
NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion
K+
enhances activity
K+
50-200 mM, Tris/HCl buffer pH 9.0
Li+
enhances activity
Li+
50-200 mM, Tris/HCl buffer pH 9.0
Mg2+
enhances activity
Mg2+
1-200 mM, Tris/HCl buffer pH 8.0
Mg2+
-
without Mg2+ low activity with coenzyme NADP
Mg2+
-
maximal activity at 20 mM
Mg2+
-
activates by 122% at 1 mM
Mn2+
inhibits enzymatic activity
Mn2+
-
maximal activity at 20 mM
Na+
enhances activity
Na+
50-200 mM, Tris/HCl buffer pH 9.0
NaCl
-
NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion
NaCl
-
2 M for activity assay
NaCl
-
stabilizes the enzyme at alkline pH and elevated temperatures
NH4+
enhances activity
NH4+
50-200 mM, Tris/HCl buffer pH 9.0
Zn2+
inhibits enzymatic activity
Zn2+
enzyme contains the catalytic zinc binding residues Cys39 and His66, no increase in activity by addition of 0.1 mM ZnCl2
additional information
enzyme does not contain weakly associated metal ions
additional information
-
extreme halophilic enzyme
additional information
-
does not require Zn2+ for enzymatic action
additional information
not affected by 0.1 mM CaCl2 and MgCl2