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0.0096 - 1.42
2-deoxy-3-oxo-6-phosphogluconate
0.55 - 1.42
2-deoxy-6-phosphogluconate
0.0048 - 0.011
6,7-dideoxy-7-phosphono-D-glucoheptonate
0.00013 - 33.7
6-phospho-D-gluconate
0.006 - 3.13
6-phosphogluconate
0.0035 - 0.03
6-phosphono-D-gluconate
0.02 - 1
ribulose-5-phosphate
0.0096
2-deoxy-3-oxo-6-phosphogluconate
-
-
0.082
2-deoxy-3-oxo-6-phosphogluconate
-
-
1.42
2-deoxy-3-oxo-6-phosphogluconate
-
-
0.55
2-deoxy-6-phosphogluconate
-
-
1.4
2-deoxy-6-phosphogluconate
-
-
1.42
2-deoxy-6-phosphogluconate
-
-
0.0048
6,7-dideoxy-7-phosphono-D-glucoheptonate
pH 8.0, 20°C
0.011
6,7-dideoxy-7-phosphono-D-glucoheptonate
pH 8.0, 20°C
0.00013
6-phospho-D-gluconate
-
-
0.0058
6-phospho-D-gluconate
pH 8.0, 25°C, mutant enzyme W104L
0.0102
6-phospho-D-gluconate
-
pH 7.6, 25°C
0.011
6-phospho-D-gluconate
-
in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C
0.011
6-phospho-D-gluconate
80°C, pH 7.5
0.0113
6-phospho-D-gluconate
pH 8.0, 25°C, wild-type enzyme
0.0121
6-phospho-D-gluconate
mutant enzyme H186A
0.019
6-phospho-D-gluconate
-
at pH 8.0 and 25°C
0.024
6-phospho-D-gluconate
mutant enzyme T262A
0.028
6-phospho-D-gluconate
wild type enzyme
0.0284
6-phospho-D-gluconate
wild type enzyme
0.0337
6-phospho-D-gluconate
pH 8.0, 25°C, wild-type enzyme
0.04
6-phospho-D-gluconate
-
dimeric enzyme with two 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication
0.042
6-phospho-D-gluconate
pH 7.3, 32°C, mutant enzyme S42T
0.0509
6-phospho-D-gluconate
pH 7.5
0.051
6-phospho-D-gluconate
at pH 7.5 and 22°C
0.052
6-phospho-D-gluconate
pH 7.3, 32°C, wild-type enzyme
0.07
6-phospho-D-gluconate
-
dimeric enzyme with one 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication
0.0822
6-phospho-D-gluconate
-
free enzyme, at pH 7.5 and 25°C
0.093
6-phospho-D-gluconate
in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C
0.095
6-phospho-D-gluconate
mutant enzyme S128A
0.107
6-phospho-D-gluconate
in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C
0.15
6-phospho-D-gluconate
mutant enzyme K260A
0.53
6-phospho-D-gluconate
mutant enzyme N187A
0.595
6-phospho-D-gluconate
-
-
1.04
6-phospho-D-gluconate
mutant enzyme Y191A
2 - 3
6-phospho-D-gluconate
mutant enzyme R287A
3.4
6-phospho-D-gluconate
mutant enzyme R446A
33.7
6-phospho-D-gluconate
pH 8.0, 25°C
0.006
6-phosphogluconate
-
-
0.0068
6-phosphogluconate
-
pH 10
0.0069
6-phosphogluconate
-
-
0.007
6-phosphogluconate
-
-
0.0096
6-phosphogluconate
-
proplastid isoenzyme
0.01
6-phosphogluconate
-
-
0.011
6-phosphogluconate
-
-
0.012
6-phosphogluconate
-
cytosolic isoenzyme
0.0135
6-phosphogluconate
-
-
0.014
6-phosphogluconate
-
60°C or 65°C
0.0149
6-phosphogluconate
-
50 mM Tris-acetate, 5-10 mM MgCl2, pH 7.7
0.015
6-phosphogluconate
-
-
0.015
6-phosphogluconate
-
cytosolic isoenzyme
0.016
6-phosphogluconate
-
-
0.016
6-phosphogluconate
-
30°C
0.0161
6-phosphogluconate
-
50 mM Tris-acetate, pH 7.7
0.0167
6-phosphogluconate
-
10 mM Tris-acetate, 80 mM KCl, pH 7.7
0.018
6-phosphogluconate
-
plastid isoenzyme
0.02
6-phosphogluconate
-
-
0.02
6-phosphogluconate
-
43°C
0.022
6-phosphogluconate
-
45°C
0.0227
6-phosphogluconate
-
-
0.023
6-phosphogluconate
-
-
0.024
6-phosphogluconate
-
-
0.02666
6-phosphogluconate
-
-
0.031
6-phosphogluconate
-
type II
0.034
6-phosphogluconate
-
-
0.0343
6-phosphogluconate
-
-
0.035
6-phosphogluconate
-
-
0.036
6-phosphogluconate
-
-
0.04
6-phosphogluconate
-
-
0.04
6-phosphogluconate
-
-
0.04 - 0.05
6-phosphogluconate
-
heterocaryon col-3-wild-type
0.0413
6-phosphogluconate
-
10 mM Tris-acetate, pH 7.7
0.043
6-phosphogluconate
-
-
0.045
6-phosphogluconate
-
-
0.05
6-phosphogluconate
-
-
0.05
6-phosphogluconate
-
50 mM Tris-acetate, 20 mM MgCl2, KCl, pH 7.7
0.05
6-phosphogluconate
-
heterocaryon col-10-wild-type
0.052
6-phosphogluconate
-
type I
0.054
6-phosphogluconate
-
-
0.054
6-phosphogluconate
-
-
0.06
6-phosphogluconate
-
col-3-col-10
0.065
6-phosphogluconate
-
-
0.068
6-phosphogluconate
-
pH 5.93
0.071
6-phosphogluconate
-
-
0.071
6-phosphogluconate
-
pH 7
0.071
6-phosphogluconate
-
source embryo
0.08
6-phosphogluconate
-
isoenzyme 2
0.085
6-phosphogluconate
-
pH 8
0.09
6-phosphogluconate
-
-
0.09
6-phosphogluconate
-
mutant col 3
0.098
6-phosphogluconate
-
-
0.1
6-phosphogluconate
-
isoenzyme 1
0.109
6-phosphogluconate
-
plastid isoenzyme
0.11
6-phosphogluconate
-
-
0.11
6-phosphogluconate
-
-
0.11
6-phosphogluconate
-
mutant col 10
0.153
6-phosphogluconate
-
pH 8.0
0.157
6-phosphogluconate
-
-
0.204
6-phosphogluconate
-
pH 9
0.22
6-phosphogluconate
-
-
0.29
6-phosphogluconate
-
-
0.313
6-phosphogluconate
-
23.5°C
0.402
6-phosphogluconate
-
source endosperm
0.64
6-phosphogluconate
-
pH 9.5
3.13
6-phosphogluconate
-
pH 9.03
0.0035
6-phosphono-D-gluconate
-
pH 7.5, 20°C
0.0053
6-phosphono-D-gluconate
pH 8.0, 20°C
0.016
6-phosphono-D-gluconate
-
pH 7.5, 20°C
0.022
6-phosphono-D-gluconate
pH 7.5, 30°C, purified native wild-type enzyme
0.0222
6-phosphono-D-gluconate
pH 7.5, 30°C, purified recombinant mutant enzyme
0.03
6-phosphono-D-gluconate
pH 8.0, 20°C
34
CO2
-
-
1.7
MgCl2
-
cytosolic isoenzyme
2.4
MgCl2
-
proplastid isoenzyme
0.01187
NAD+
mutant enzyme A30D/R31I/T32I, at pH 7.5 and 50°C
0.0119
NAD+
recombinant His-tagged mutant A30D/R31I/T32I, pH 7.5, 50°C
0.127
NAD+
mutant enzyme A30E/R31I/T32D, at pH 7.5 and 50°C
0.127
NAD+
recombinant His-tagged mutant A30E/R31I/T32D, pH 7.5, 50°C
0.354
NAD+
mutant enzyme R31I, at pH 7.5 and 50°C
0.354
NAD+
recombinant His-tagged mutant R31I, pH 7.5, 50°C
0.362
NAD+
mutant enzyme R31I/T32G, at pH 7.5 and 50°C
0.362
NAD+
recombinant His-tagged mutant R31I/T32G, pH 7.5, 50°C
0.38
NAD+
-
in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C
0.404
NAD+
mutant enzyme A30C/R31I/T32K, at pH 7.5 and 50°C
0.404
NAD+
recombinant His-tagged mutant A30C/R31I/T32K, pH 7.5, 50°C
0.605
NAD+
mutant enzyme R31T, at pH 7.5 and 50°C
0.605
NAD+
recombinant His-tagged mutant R31T, pH 7.5, 50°C
1.397
NAD+
wild type enzyme, at pH 7.5 and 50°C
1.397
NAD+
recombinant His-tagged wild-type enzyme, pH 7.5, 50°C
2.5
NAD+
mutant enzyme N32E/R33I/T34I, at pH 7.5 and 70°C
3.9
NAD+
mutant enzyme N32D/R33I/T34I, at pH 7.5 and 70°C
4
NAD+
mutant enzyme N32D, at pH 7.5 and 70°C
4.3
NAD+
wild type enzyme at pH 7.5 and 70°C
4.46
NAD+
pH 7.3, 32°C, wild-type enzyme
6.6
NAD+
pH 7.3, 32°C, mutant enzyme S42T
7.5
NAD+
mutant enzyme N32D/R33L/T34S, at pH 7.5 and 70°C
0.00088
NADP+
-
-
0.002
NADP+
wild type enzyme
0.002
NADP+
mutant enzyme H186A
0.0034
NADP+
-
free enzyme, at pH 7.5 and 25°C
0.0041
NADP+
-
cytosolic isoenzyme
0.0042
NADP+
mutant enzyme T262A
0.0046
NADP+
pH 7.5, 30°C, purified native wild-type enzyme
0.0048
NADP+
pH 8.0, 25°C, mutant enzyme W104L
0.005
NADP+
wild type enzyme
0.005
NADP+
wild type enzyme, at pH 7.5 and 70°C
0.0052
NADP+
-
at pH 8.0 and 25°C
0.0054
NADP+
-
proplastid isoenzyme
0.0054
NADP+
mutant enzyme S128A
0.00571
NADP+
-
10 mM Tris-acetate, 10 mM MgCl2, pH 7.7
0.0059
NADP+
pH 7.5, 30°C, purified recombinant mutant enzyme
0.006
NADP+
-
source endosperm
0.00631
NADP+
-
10 mM Tris-acetate, 80 mM KCl, pH 7.7
0.0064
NADP+
mutant enzyme M13C
0.00676
NADP+
-
50 mM Tris-acetate, pH 7.7
0.0069
NADP+
pH 8.0, 25°C, wild-type enzyme
0.008
NADP+
-
cytosolic isoenzyme
0.00861
NADP+
-
50 mM Tris-acetate, 20 mM MgCl2, pH 7.7
0.00893
NADP+
-
10 mM Tris-acetate, pH 7.7
0.009
NADP+
pH 8.0, 25°C, wild-type enzyme
0.01
NADP+
-
wild-type, mutant col 10, heterocaryon col-10-wild-type
0.01
NADP+
-
in 50 mM HEPES buffer (pH 7.5) containing 5 mM Mg2+, 0.5 mM Mn2+, and 0.5 mg bovine serum albumin, at 80°C
0.011
NADP+
-
plastid isoenzyme
0.0139
NADP+
wild type enzyme, at pH 7.5 and 50°C
0.0139
NADP+
recombinant His-tagged wild-type enzyme, pH 7.5, 50°C
0.014
NADP+
mutant enzyme Y191A
0.015
NADP+
-
heterocaryon col 3-wild-type
0.015
NADP+
pH 7.3, 32°C, mutant enzyme S42T
0.016
NADP+
mutant enzyme K260A
0.018
NADP+
-
heterocaryon col-3-col-10
0.018
NADP+
at pH 7.5 and 22°C
0.019
NADP+
mutant enzyme M13F
0.019
NADP+
mutant enzyme M13Q
0.019
NADP+
D4ZTT4
pH 7.3, 32°C, wild-type enzyme
0.02
NADP+
-
type I and II
0.023
NADP+
-
plastid isoenzyme
0.025
NADP+
-
source of enzyme: peak I
0.026
NADP+
mutant enzyme M13V
0.0265
NADP+
mutant enzyme R31I, at pH 7.5 and 50°C
0.0265
NADP+
recombinant His-tagged mutant R31I, pH 7.5, 50°C
0.0298
NADP+
mutant enzyme N187A
0.03
NADP+
-
mutant col-3
0.03
NADP+
-
source of enzyme: whole cells
0.032
NADP+
-
source embryo
0.032
NADP+
mutant enzyme M13I
0.033
NADP+
-
source of enzyme: peak II
0.0355
NADP+
mutant enzyme R31T, at pH 7.5 and 50°C
0.0355
NADP+
recombinant His-tagged mutant R31T, pH 7.5, 50°C
0.042
NADP+
D4ZTT4
pH 7.3, 32°C, mutant enzyme T35S
0.049
NADP+
in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C
0.058
NADP+
pH 7.3, 32°C, wild-type enzyme
0.064
NADP+
mutant enzyme R446A
0.073
NADP+
mutant enzyme R287A
0.1044
NADP+
mutant enzyme R31I/T32G, at pH 7.5 and 50°C
0.1044
NADP+
recombinant His-tagged mutant R31I/T32G, pH 7.5, 50°C
0.118
NADP+
in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C
0.228
NADP+
mutant enzyme A30D/R31I/T32I, at pH 7.5 and 50°C
0.228
NADP+
recombinant His-tagged mutant A30D/R31I/T32I, pH 7.5, 50°C
0.66
NADP+
mutant enzyme A30E/R31I/T32D, at pH 7.5 and 50°C
0.66
NADP+
recombinant His-tagged mutant A30E/R31I/T32D, pH 7.5, 50°C
0.698
NADP+
mutant enzyme A30C/R31I/T32K, at pH 7.5 and 50°C
0.698
NADP+
recombinant His-tagged mutant A30C/R31I/T32K, pH 7.5, 50°C
2.3
NADP+
mutant enzyme N32D, at pH 7.5 and 70°C
70
NADP+
mutant enzyme N32D/R33I/T34I, at pH 7.5 and 70°C
79
NADP+
mutant enzyme N32E/R33I/T34I, at pH 7.5 and 70°C
100
NADP+
Km above 100 mM, mutant enzyme N32D/R33L/T34S, at pH 7.5 and 70°C
0.00022
NADPH
-
-
0.02
ribulose-5-phosphate
-
-
1
ribulose-5-phosphate
-
-
1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)
results (
results (
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