1.1.1.44: phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) This is an abbreviated version! For detailed information about phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating), go to the full flat file .
Reaction
6-phospho-D-gluconate +
NADP+ =
D-ribulose 5-phosphate +
CO2 +
NADPH +
H+
Synonyms 6-GPD, 6-P-gluconate dehydrogenase, 6-Pgd, 6-PGDH, 6-PGDHase, 6-phospho-D-gluconate dehydrogenase, 6-phospho-D-gluconate-NADP+ oxidoreductase, decarboxylating, 6-phosphogluconate dehydrogenase, 6-phosphogluconate dehydrogenase (decarboxylating), 6-phosphogluconate dehydrogenase 1, 6-phosphogluconate dehydrogenase Gnd1, 6-phosphogluconate dehydrogenase, decarboxylating, 6-phosphogluconate-dehydrogenase, 6-phosphogluconate:NADP oxidoreductase, 6-phosphogluconic carboxylase, 6-phosphogluconic dehydrogenase, 6-phosphonogluconate dehydrogenase, 6PDH, 6PG DH, 6PGD, 6PGDH, 6PGDH/Gnd1, 6PGDH1, 6PGDH2, 6PGDH3, At1G64190, At3g02360, At5g41670, D-gluconate-6-phosphate dehydrogenase, GCG1, gnd, GND1, Gnd1p, Gnd2p, GNTZII, LlPDH, Moth_1283, Os6PGDH1, Os6PGDH2, p6PGDH, peroxisomal 6-phosphogluconate dehydrogenase, Pgd, PGD1, PGD2, PGD3, phosphogluconic acid dehydrogenase, TM0438, YpjI, zwf3
ECTree
Cloned
Cloned on EC 1.1.1.44 - phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)
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6-phosphogluconate dehydrogenase (encoded by zwf3) is overexpressed with opc encoding a positive allosteric effector essential for Zwf activity in Streptomyces lividans TK24
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6PGDH cDNA encodes a protein with 94% sequence identity with the sheep liver enzyme
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amino acid sequence of the sheep liver enzyme deduced from cDNA clones
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cloning of the LT2 gnd gene
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DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, functional overexpression of soluble His-tagged wild-type and mutant enzymes in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) pLysS cells
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli M15 cells
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expressed in Escherichia coli Rosetta2 (DE3) cells
expressed in Escherichia coli strain M15
expressed in Escherichia coli strain M15[pREP4]
expression in Escherichia coli
expression of His-tagged enzymes in Escherichia coli M15 cells
gene 6pgdh, recombinant expression in Escherichia coli TOP10, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene 6pgdh, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
glutathione S-transferase (GST)-tagged enzyme is expressed in Escherichia coli
glutathione S-transferase (GST)-tagged Sy6PGDH is expressed in Escherichia coli
mRNA encoding the 51-kDa subunit of 6-phosphogluconate dehydrogenase from sheep liver reverse transcribed and amplified resulting cDNA subcloned into pQE-30 and overproduced in Escherichia coli M15
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overexpression in Escherichia coli
overexpression in Escherichia coli M15
the A134T mutant is expressed in Escherichia coli BL21 (DE3) cells
the Y378C mutant is expressed in Escherichia coli BL21 (DE3) cells
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expressed in Escherichia coli
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expressed in Escherichia coli
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expressed in Escherichia coli
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expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli
expression of His-tagged enzymes in Escherichia coli M15 cells
expression of His-tagged enzymes in Escherichia coli M15 cells
glutathione S-transferase (GST)-tagged Sy6PGDH is expressed in Escherichia coli
glutathione S-transferase (GST)-tagged Sy6PGDH is expressed in Escherichia coli
D4ZTT4
overexpression in Escherichia coli
overexpression in Escherichia coli
overexpression in Escherichia coli M15
overexpression in Escherichia coli M15