Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.1.41: isocitrate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about isocitrate dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.41

Reaction

isocitrate
+
NAD+
=
2-oxoglutarate
 +
CO2
 +
NADH
 +
H+

Synonyms

At2g17130, At4g35260, At4g35650, At5g03290, AtIDH I, AtIDH II, AtIDH V, AtIDH VI, beta-decarboxylating dehydrogenase, beta-ketoglutaric-isocitric carboxylase, CaIDH, CcIDH, hNAD-IDHalpha, homodimeric NAD+-linked isocitrate dehydrogenase, Icd-2, ICDH, ICDH1, IDH, IDH I, IDH II, IDH III, IDH IV, IDH V, IDH-50, IDH-I, IDH-II, IDH-V, IDH1, IDH2, IDH3, IDH3A, IDH3alpha, IDH5, IDHa, isocitrate dehydrogenase, isocitrate dehydrogenase 2, isocitrate dehydrogenase 3alpha subunit, isocitrate-homoisocitrate dehydrogenase, isocitric acid dehydrogenase, isocitric dehydrogenase, McIDH, mIDH, MiIDH, mitochondrial NAD-dependent isocitrate dehydrogenase, monomeric NAD-IDH, NAD dependent isocitrate dehydrogenase, NAD isocitrate dehydrogenase, NAD isocitric dehydrogenase, NAD(P)+-dependent isocitrate dehydrogenase, NAD+-dependent ICDH, NAD+-dependent IDH, NAD+-dependent isocitrate dehydrogenase, NAD+-dependent isocitrate dehydrogenase 1, NAD+-dependent isocitrate dehydrogenase 3, NAD+-dependent type 1 IDH, NAD+-IDH, NAD+-specific ICDH, NAD+-specific IDH, NAD+-specific isocitrate dehydrogenase, NAD-Dependent IDH, NAD-dependent isocitrate dehydrogenase, NAD-ICDH, NAD-IDH, NAD-isocitrate dehydrogenase, NAD-linked isocitrate dehydrogenase, NAD-specific isocitrate dehydrogenase, NADH-IDH, OlIDH, OtIDH, PF0202, PH1722, Rv0066c, threo-DS-isocitrate:NAD+ oxidoreductase (decarboxylating), type 1 isocitrate dehydrogenase, type II homodimeric NAD-IDH, type II NAD+-specific isocitrate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.41 isocitrate dehydrogenase (NAD+)

Crystallization

Crystallization on EC 1.1.1.41 - isocitrate dehydrogenase (NAD+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant isocitrate dehydrogenase complexed with NAD+ and citrate solved to a resolution of 1.9 A, hanging drop vapor diffusion method
-
crystallization in artificial mother liquor supplemented with 100 mM NAD+
-
alphabeta heterodimer with the alpha subunit in apo form and in Ca2_-bound, NAD-bound, and NADH-bound forms, hanging drop vapor diffusion method, using either containing 8% (v/v) tacsimate (pH 6.0) and 20% (w/v) PEG 3350 or 0.2 M calcium acetate (pH 7.5) and 20% (w/v) PEG 3350
alphagamma heterodimer bound with an Mg2+ and an NADH at the active site and an NADH at the allosteric site, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 50 mM MgCl2, and 30% (w/v) PEGMME 550
alphagamma heterodimer with the active site bound with either Mg2+ alone or Mg2+ and ATP, and the allosteric site bound with Mg2+, citrate and ATP, hanging drop vapor diffusion method, using 0.2 M sodium citrate, pH 8.0, and 20% (w/v) PEG3350 or 0.2 M sodium citrate, pH 5.5, and 24% (v/v) PEG400
isoform IDH3 containing a beta mutant in apo form, hanging drop vapor diffusion method, using 0.05M NH4Cl, 0.05 Bis-Tris (pH 6.5), and 30% (w/v) pentaerythritol ethoxylate
sitting drop vapour diffusion method, using 3-5% (w/v) PEG 20000, 0.1 M MES pH 5.7 and 0.2 M CaCl2
-
sitting drop vapor diffusion method, using 200 mM NaCl, 100 mM sodium HEPES (pH 7.5), and 25% (w/v) PEG 4000
-
hanging drop vapor diffusion method
-
sitting drop vapor diffusion method, using 100 mM HEPES-NaOH buffer, pH 7.2, 200 mM NaCl and 22% (w/v) PEG6000
-