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1.1.1.4: (R,R)-butanediol dehydrogenase

This is an abbreviated version!
For detailed information about (R,R)-butanediol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.4

Reaction

(R,R)-butane-2,3-diol
+
NAD+
=
(R)-acetoin
 +
NADH
 +
H+

Synonyms

(2R,3R)-2,3-BDH, (2R,3R)-2,3-butanediol dehydrogenase, (R)-2,3-butanediol dehydrogenase, (R)-diacetyl reductase, (R,R)-butane-2,3-diol dehydrogenase, 1-amino-2-propanol dehydrogenase, 1-amino-2-propanol oxidoreductase, 2,3-BDH/AR, 2,3-butanediol dehydrogenase, 2,3-butanediol dehydrogenase/acetoin reductase, 2R,3R-2,3-BD dehydrogenase, 2R-3R-BDH, acetoin reductase, acetoin/diacetyl reductase, ADH-9, aminopropanol oxidoreductase, AR/BDH, BaBdhA, BcBDH, BDH, BDH1, Bdh1p, BDH2, Bdh2p, BDH99::67, BdhA, BtBDH, ButA, butanediol dehydrogenase, ButB, butylene glycol dehydrogenase, butyleneglycol dehydrogenase, D-(-)-butanediol dehydrogenase, D-1-amino-2-propanol dehydrogenase, D-1-amino-2-propanol:NAD+ oxidoreductase, D-2,3-BD dehydrogenase, D-aminopropanol dehydrogenase, dehydrogenase, D-aminopropanol, dehydrogenase, D-butanediol, DHAD, diacetyl reductase (acetoin), EC 1.1.1.74, GDH, GldA, meso-2,3-butanediol dehydrogenase, meso-BDH, MF996569, More, NAD(H)-dependent 2,3-butanediol dehydrogenase, NADH-dependent 2,3-butanediol dehydrogenase, PA4153, PB24_3312, R,R-2,3-butanediol dehydrogenase/meso-2,3-butanediol dehydrogenase/diacetyl reductase, R,R-BD-DH, R,R-BDH, R-selective 2,3-butanediol dehydrogenase, SLAC

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.4 (R,R)-butanediol dehydrogenase

Cloned

Cloned on EC 1.1.1.4 - (R,R)-butanediol dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21 (DE3)
-
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Serratia marcescens MG1 slaC mutant strain
-
expression in Escherichia coli
gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli
gene BDH1, cloning from Saccharomyces cerevisiae strains YBA_08 (denoted Y) and BY4742 (denoted B), DNA and amino acid sequence determination and analysis and phylogenetic tree analysis, subcloning in to Escherichia coli strain DH5alpha and functional overexpression in Saccharomyces cerevisiae strain INVSc1, recombinant expression of His-tagged enzymes originnating from both strains
gene BDH1, construction of plasmid B1ZF-loxP-KanMX-loxPPGK1p-BDH1-PGK1t-B1ZR in Escherichia coli strain DH5alpha, and recombinant overexpression in Saccharomyces uvarum strain WY1 resulting in strain WY1-1, overexpression of gene BDH2 from Saccharomyces uvarum (WY1-2), method overview. BDH1 and BDH2 overexpression in one strain, named WY1-12, is constructed on the basis of WY1-2, that is, the strain WY1-2 is transformed into plasmid Yep-KPB1 after excising KanMX, real-time PCR expression analysis
-
gene bdhA, recombinant eenzyme expression in non-natural AC and 2,3-BD producing Corynebacterium crenatum derived from wild-type strain SYPA5-5, coexpression of 2,3-BD biosynthesis pathway genes alsS, alsD, and bdhA
gene bdhA, recombinant expression of His-tagged enzyme BDHA in Escherichia coli strain BL21 (DE3), co-expression with polyol dehydrogenase (GoSCR) from Gluconobacter oxydans for asymmetric reduction of 2-hydroxyacetophenone (2-HAP) to (R)-1-phenyl-1,2-ethanediol ((R)-PED) or (S)-1-phenyl-1,2-ethanediol ((S)-PED)
gene bdhA, recombinant expression of N-terminally StrepII-tagged enzyme in Escherichia coli strain BL21(DE3)
gene bdhA, the the BDH gene sequence is cloned from genomic DNA from strain 168, the BDH encoded by the gene bdhA is responsible for catalysing the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol
gene gldA, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3)
-
gene mbd1, DNA and amino acid sequence determination and analysis, phylogenetic analysis
genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview
open reading frame (ORF) scaffold99_orf00067, DNA and amino acid sequence determination and analysis, phylogenetic analysis
overexpression in Saccharomyces cerevisiae
-
phylogenetic analysis
recombinant enzyme expression in Saccharomyces cerevisiae strain YPH499, co-expression with alpha-acetolactate synthase from Bacillus subtilis, alpha-acetolactate decarboxylase from Bacillus amyloliquefaciens, and NADH oxidase from Lactococcus lactis
subcloning of mutant proteins via plasmids in Bacillus subtilis strain WN1038
-