1.1.1.346: 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)
This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming), go to the full flat file.
Reaction
Synonyms
2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconate reductase I, 2,5-diketo-D-gluconate reductase II, 2,5-diketo-D-gluconate-reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-D-gluconic acid reductase A, 2,5-DKG reductase, 2,5-DKGR A, 2,5-DKGR B, 2,5-DKGRA, 2,5DKGR, 25DKG reductase, 25DKGR-A, 2KR, AKR5C, CTATCC11996_22452, dkgA, dkgB, Dkr, YqhE, YqhE reductase
ECTree
1.1.1.346 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)Advanced search results
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results (Substrates Products
Substrates Products on EC 1.1.1.346 - 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,5-didehydro-D-gluconate + NADPH + H+
5-dehydro-D-gluconate + NADP+
Brevibacterium ketosoreductum
-
100% activity
-
-
?
2-dehydro-D-gluconate + NADPH + H+
D-gluconate + NADP+
Brevibacterium ketosoreductum
-
11.8% activity compared to 2,5-didehydro-D-gluconate
-
-
?
2-dehydro-L-gulonate + NADPH + H+
L-idonate + NADP+
Brevibacterium ketosoreductum
-
10.8% activity compared to 2,5-didehydro-D-gluconate
-
-
?
5-dehydro-D-fructose + NADPH + H+
L-sorbose + NADP+
-
isoforms 2,5-diketo-D-gluconate reductase I and II show 150% and 13% activity, respectively, compared to 2,5-didehydro-D-gluconate
-
-
?
ethyl 2-acetylpent-4-enoate + NADPH + H+
ethyl (2R)-2-[(1S)-1-hydroxyethyl]pent-4-enoate + NADP+
-
250% activity compared to ethyl 2-methylacetoacetate
-
-
?
ethyl 2-ethyl-3-oxobutanoate + NADPH + H+
ethyl (2R,3S)-2-ethyl-3-hydroxybutanoate + NADP+
-
120% activity compared to ethyl 2-methylacetoacetate
-
-
?
ethyl 2-methylacetoacetate + NADH + H+
ethyl (2R)-methyl-(3S)-hydroxybutanoate + NAD+
-
7% activity with NADH compared to NADPH
-
-
?
ethyl 2-methylacetoacetate + NADPH + H+
ethyl (2R)-methyl-(3S)-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl acetoacetate + NADPH + H+
ethyl (3S)-3-hydroxybutanoate + NADP+
-
53% activity compared to ethyl 2-methylacetoacetate
-
-
?
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-L-gulonate + NADP+
-
-
-
-
?
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-L-gulonate + NADP+
-
-
-
?
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-L-gulonate + NADP+
-
100% activity
-
-
?
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-L-gulonate + NADP+
-
a reduction of substrate by NADPH is highly preferred
-
-
r
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-L-gulonate + NADP+
-
isoform DKGR B exhibits 66fold higher specific activity toward 2,5-didehydro-D-gluconate than isoform DKGR A
-
-
?
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-L-gulonate + NADP+
-
a reduction of substrate by NADPH is highly preferred
-
-
r
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-L-gulonate + NADP+
-
100% activity
-
-
?
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-L-gulonate + NADP+
NADPH is the preferred electron donor
-
-
?
2-dehydro-L-gulonate + NADP+
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
-
?
2-dehydro-L-gulonate + NADP+
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
-
?
2-dehydro-L-gulonate + NADP+
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
-
?
2-dehydro-L-gulonate + NADP+
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
-
?
2-dehydro-L-gulonate + NADP+
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
-
r
additional information
?
-
Brevibacterium ketosoreductum
-
no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate
-
-
?
additional information
?
-
Brevibacterium ketosoreductum ATCC 21914
-
no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate
-
-
?
additional information
?
-
-
the enzyme catalyzes degradation of estradiol, oestrone, testosterone, and methyltestosterone, overview
-
-
?
additional information
?
-
-
the enzyme catalyzes degradation of estradiol, oestrone, testosterone, and methyltestosterone, overview
-
-
?
additional information
?
-
-
no activity with 2-dehydro-L-gulonate, 2-dehydro-D-gluconate, 5-dehydro-D-gluconate, D-fructose, and L-sorbose
-
-
?
additional information
?
-
-
the wild type enzyme shows no activity with NADH
-
-
?
additional information
?
-
-
no activity with 2-dehydro-L-gulonate, 2-dehydro-D-gluconate, 5-dehydro-D-gluconate, D-fructose, and L-sorbose
-
-
?
additional information
?
-
-
2-keto-D-gluconate, 2-keto-L-gulonate, 5-keto-D-gluconate, D-fructose, and L-sorbose are inactive when assayed at pH 6.0 in the presence of NADH or NADPH
-
-
?
additional information
?
-
2-keto-D-gluconate, 2-keto-L-gulonate, 5-keto-D-gluconate, D-fructose, and L-sorbose are inactive when assayed at pH 6.0 in the presence of NADH or NADPH
-
-
?
