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1.1.1.345: D-2-hydroxyacid dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about D-2-hydroxyacid dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.345

Reaction

an (R)-2-hydroxycarboxylate
+
NAD+
=
a 2-oxocarboxylate
+
NADH
+
H+

Synonyms

D-2-HADH, D-2-hydroxyacid dehydrogenase, D-2-hydroxyisocaproate dehydrogenase, D-HicDH, D-isomer specific 2-hydroxyacid dehydrogenase, D-isomer specific NAD+-dependent 2-hydroxyacid dehydrogenase, D-isomer-specific 2-hydroxyacid dehydrogenase, D-lactate dehydrogenase, D-lactate:NAD+ oxidoreductase, D-malate dehydrogenases, D-mandelate dehydrogenase, D-ManDH1, D-ManDH2, D-MDH, D2-HDH, HdhD, Ldb1010, ldh1837, LEUM_1837, ManDH2, NAD-dependent D-2-hydroxyacid dehydrogenase, PanE, R-HicDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.345 D-2-hydroxyacid dehydrogenase (NAD+)

KM Value

KM Value on EC 1.1.1.345 - D-2-hydroxyacid dehydrogenase (NAD+)

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.3
(R)-2-hydroxy-4-methylpentanoate
-
pH 7.0, temperature not specified in the publication
0.76 - 0.9
(R)-mandelate
1.26
2-formylbutanethioate
-
pH 7.0, 37°C
2.7
2-hydroxyhexanoate
-
pH 7.0, temperature not specified in the publication
1.6
2-Hydroxyoctanoate
-
pH 7.0, temperature not specified in the publication
3.3
2-hydroxypentanoate
-
pH 7.0, temperature not specified in the publication
3.4 - 5.7
2-oxo-3-phenylpropanoate
4 - 9.5
2-oxobutanoate
0.36 - 28
2-oxobutyrate
2.4 - 40
2-oxocaproate
0.02 - 1.5
2-Oxohexanoate
0.3 - 71
2-oxoisocaproate
0.29 - 46
2-oxoisovalerate
0.21
2-oxomethylvalerate
-
at pH 7.0 and 37°C
0.12
2-oxooctanoate
-
pH 7.0, temperature not specified in the publication
0.057 - 0.4
2-oxopentanoate
0.53 - 62
2-oxovalerate
0.6
3-(4-hydroxyphenyl)-2-oxopropanoate
-
pH 7.0, temperature not specified in the publication
2.9
3-hydroxypyruvate
-
in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C
0.15 - 0.29
3-methyl-2-oxobutanoate
0.21
3-methyl-2-oxopentanoate
-
pH 7.0, 37°C
0.022 - 0.4
4-methyl-2-oxopentanoate
1.26
4-methylthio-2-oxobutanoate
-
at pH 7.0 and 37°C
1.5
benzoylformate
0.14
D-lactate
-
in 0.1 M acetate buffer, pH 5.0, 4 M NaCl, at 52°C
3
D-mandelate
-
at pH 7.0 and 37°C
3
DL-2-hydroxyisocaproate
-
at pH 7.0 and 37°C
0.5
NAD+
pH 7.0, 25°C
0.01 - 0.14
NADH
0.25 - 0.26
phenylglyoxylate
0.031 - 20
phenylpyruvate
0.56 - 1.2
pyruvate
additional information
additional information
Michaelis-Menten kinetics, recombinant enzyme
-