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1.1.1.34: hydroxymethylglutaryl-CoA reductase (NADPH)

This is an abbreviated version!
For detailed information about hydroxymethylglutaryl-CoA reductase (NADPH), go to the full flat file.

Word Map on EC 1.1.1.34

Reaction

(R)-mevalonate
+
CoA
 + 2 NADP+ =
(S)-3-hydroxy-3-methylglutaryl-CoA
 + 2 NADPH + 2 H+

Synonyms

3-hydroxy-3-methyl-glutaryl CoA reductase, 3-hydroxy-3-methylglutaryl Ccoenzyme A reductase 1, 3-hydroxy-3-methylglutaryl co-enzyme A reductase, 3-hydroxy-3-methylglutaryl CoA reductase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, 3-hydroxy-3-methylglutaryl coenzyme A reductase 1, 3-hydroxy-3-methylglutaryl-CoA reductase, 3-hydroxy-3-methylglutaryl-CoA reductase (NADPH), 3-hydroxy-3-methylglutaryl-CoA reductase 1, 3-hydroxy-3-methylglutaryl-coenzyme A reductase, 3-hydroxy-3-methylglutaryl-coenzyme A reductase 5, 3-hydroxy-3-metylglutaryl coenzyme A reductase, 3-hydroxymethylglutaryl coenzyme A reductase, acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase, beta-hydroxy-beta-methylglutaryl coenzyme A reductase, beta-hydroxy-beta-methylglutaryl-Co A reductase, EuHMGR, HMG CoA reductase, HMG-CoA reductase, HMG-CoA-R, HMG-CoAR, HMG1, HMG2, HMG2.2, Hmg2p, HMG3.3, HMGCoA reductase, HMGCoA reductase-mevalonate:NADP-oxidoreductase (acetylating CoA), HMGCoAR, HMGCR, HMGCR1, HMGR, HMGR1, HMGR1S, HMGR2, HMGR3, HMGR5, hydroxy-3-methylglutoryl-Coenzyme A reductase, hydroxymethylglutaryl CoA reductase (NADPH), hydroxymethylglutaryl-coenzyme A reductase (reduced nicotinamide adenine dinucleotide phosphate), mevalonate:NADP+ oxidoreductase (acetylating CoA), microsomal HMG-CoA reductase, NADPH-hydroxymethylglutaryl-CoA reductase, S-3-hydroxy-3-methylglutaryl-CoA reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH)

Purification

Purification on EC 1.1.1.34 - hydroxymethylglutaryl-CoA reductase (NADPH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3.8fold, recombinant enzyme from Escherichia coli
-
4380fold to homogeneity from microsomes by solubilization with trypsin, and affinity chromatography on 2',5'-ADP-resin and HMG-CoA-hexane resin
-
anti-FLAG-tag magnetic bead chromatography
isozymes Hmg1 and Hmg2 partially by subcellular fractionation
-
native enzyme by ultracentrifugation
-
native soluble isozyme HMGR1 from mandibular organ by ultracentrifugation, anion exchange chromatography, ultrafiltration and gel filtration, recombinant enzyme from Sf9 insect cells by nickel affinity chromatography
Ni-NTA column chromatography
partially, preparation of microsomes and solubilization by trypsin
-
recombinant His-tagged catalytic domain from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant solubilized enzyme from Escherichia coli strain BL21(DE3) Rosetta by nickel affinity chromatography and dialysis