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1.1.1.318: eugenol synthase

This is an abbreviated version!
For detailed information about eugenol synthase, go to the full flat file.

Word Map on EC 1.1.1.318

Reaction

eugenol
+
a carboxylate
 +
NADP+
=
a coniferyl ester
 +
NADPH
 +
H+

Synonyms

CbEGS1, CbEGS2, chavicol/eugenol synthase, DcE(I)GS1, DcEGS1, EGS, EGS1, EGS2, eugenol synthase 1, eugenol synthase 2, eugenol synthase 3, eugenol synthase 4, eugenol synthase 5, eugenol synthase 6, eugenol synthase 7, eugenol synthase 8, eugenol/isoeugenol synthase, IEGS1, IEGS2, LtCES1, More, ObEGS1, ObEGS2, ObEGS3, ObEGS4, ObEGS5, ObEGS6, ObEGS7, ObEGS8, PhEGS1, RcEGS1, RhEGS1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.318 eugenol synthase

Cloned

Cloned on EC 1.1.1.318 - eugenol synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in GST-tagged Escherichia coli strain BL21
-
DNA and amino acid sequence determination and analysis, sequence comparison, quantitative expression analysis
-
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
-
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of nontagged and of His-tagged CbEGS1 and CbEGS2 in Escherichia coli
-
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative expression analysis
EGS1 expression in Fragaria x ananassa cv. Elsanta via transfection with Agrobacterium tumefaciens strain AGL0, downregulation of chalcone synthase via antisense construct in parallel
gene DcEGS1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli
gene EGS1, cloning from petals of Rosa chinensis cv. Old Blush, DNA and amino acid sequence determination, recombinant overexpression of His-tagged enzyme in Escherichia coli from pTRV2 vector
gene EGS1, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter. Analysis of ObEGS1 and ObEGS4 endogenous promoters shows the presence of different cis regulatory elements, expression pattern. An AC-II element is found in ObEGS1 promoter and a MYB biding site (MBS) is found in ObEGS4
gene egs1, quantitative real-time PCR enzyme expression analysis
gene EGS1, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3). Transient coexpression of ObCAAT1 and/o ObCAAT2 with ObEGS1 under control of CMV 35S promoter in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection, the transgenic leaves produce eugenol only when external coniferyl alcohol is added
gene EGS2, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS3, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS4, genotyping and quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter. Analysis of ObEGS1 and ObEGS4 endogenous promoters shows the presence of different cis regulatory elements, expression pattern. An AC-II element is found in ObEGS1 promoter and a MYB biding site (MBS) is found in ObEGS4
gene EGS5, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS6, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS7, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS8, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter