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1.1.1.28: D-lactate dehydrogenase

This is an abbreviated version!
For detailed information about D-lactate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.28

Reaction

(R)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

D-(-)-lactate dehydrogenase, D-(-)-lactate dehydrogenase (NAD), D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein, D-lactate dehydrogenase, D-lactic acid dehydrogenase, D-lactic dehydrogenase, D-LDH, D-LDH-like enzyme, D-LDH0653, D-LDH1, D-LDH2, D-LDH3, D-LDH82319, D-nLDH, D-specific lactic dehydrogenase, dehydrogenase, D-lactate, DLD1, DLDH, DLDH744, ECBD_2243, ECLDH, FD35_GL001981, Fermentative lactate dehydrogenase, FN0511, FNLDH, lactic acid dehydrogenase, ldb0101, Ldb1010, LDH, LdhA, ldhd, LDHD1, LDHD2, LDHD3, LdhTi, Ljd-LDH, MGG_01202, NAD-dependent D-lactate dehydrogenase, PA0927, PALDH, Respiratory D-lactate dehydrogenase, SO_0968, tp0037, WP_013906894

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.28 D-lactate dehydrogenase

Temperature Stability

Temperature Stability on EC 1.1.1.28 - D-lactate dehydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 55
-
thermal stability of the D-LDH isozymes, pH 7.0, with sodium pyruvate as a substrate, profiles, overview
30 - 40
-
the enzyme is significantly stable below 30°C, but the activity decreases significantly above 40°C
30 - 45
-
87% activity remaining after 4 h at 30°C, higher loss of activity above 45°C
35
-
stable below, Tris-HCl, pH 8.5
39
purified recombinant His-tagged enzyme, pH 8.0, stable up to
48.7
mutant H296A, melting temperature
49
purified recombinant His-tagged enzyme, pH 8.0, stable up to
49.1
wild-type, melting temperature
49.3
wild-type, melting temperature, presence of 20 mM L-lactate
50.1
wild-type, melting temperature, presence of 20 mM D-lactate
53.4
wild-type, melting temperature, presence of 5 mM NAD+
53.5
mutant Y101A, melting temperature
55.8
mutant R235A, melting temperature
57
purified recombinant His-tagged enzyme, pH 5.0, stable up to