1.1.1.274: 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)
This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming), go to the full flat file.
Word Map on EC 1.1.1.274
-
1.1.1.274
-
2,5-diketo-d-gluconic
-
corynebacterium
-
2-keto-l-gulonic
-
nadph-dependent
-
aldo-keto
-
l-ascorbic
-
d-glucose
-
synthesis
-
citrinum
-
penicillium
-
activity-stained
-
aldose
-
cofactor-binding
-
reductases
-
biocatalysis
-
erwinia
-
error-prone
-
stereo-specific
-
ascorbate
-
enantioselectivity
-
two-phase
-
pantoea
-
citrea
-
biotechnology
- 1.1.1.274
-
2,5-diketo-d-gluconic
- corynebacterium
-
2-keto-l-gulonic
-
nadph-dependent
-
aldo-keto
-
l-ascorbic
- d-glucose
- synthesis
- citrinum
-
penicillium
-
activity-stained
- aldose
-
cofactor-binding
- reductases
-
biocatalysis
- erwinia
-
error-prone
-
stereo-specific
- ascorbate
-
enantioselectivity
-
two-phase
-
pantoea
- citrea
- biotechnology
Reaction
Synonyms
2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-gluconate reductase, 2,5-DKG, 2,5-DKG reductase, 2,5-DKGR, 2,5DKGR, beta-keto ester reductase, CTATCC11996_22452, Dkr, YafB, YqhE, YqhE reductase
ECTree
Advanced search results
Application
Application on EC 1.1.1.274 - 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
biotechnology
enzyme is a target for the construction of a NADH-utilizing mutant strain in the industrial production of vitamin C
synthesis
enzyme may be useful for the synthesis of the ascorbate precursor 2-keto-L-gulonate
synthesis
enzyme can be used in the industrial production of vitamin C
synthesis
enzymatic production of the vitamin C precursor 2-keto-L-gulonate (2-KLG) from 2,5-diketo-D-gluconate (2,5-DKG) by coupling 2,5-diketo-D-gluconic acid reductase via its coenzyme to glucose dehydrogenase. The bienzymatic process shows complicated inhibition patterns caused by reaction products, NADP+ and NADPH. The key parameters for a fast and efficient conversion are the NADP(H) concentration, the volumetric activity of 2,5-DKG reductase, the ratio of synthetic enzyme activity to regenerate enzyme activity and the glucono-1,5-lactone concentration. By modeling the space-time yield of the process is nearly doubled and the coenzyme concentration reduced threefold