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1.1.1.198: (+)-borneol dehydrogenase

This is an abbreviated version!
For detailed information about (+)-borneol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.198

Reaction

(+)-borneol
+
NAD+
=
(+)-Camphor
+
NADH
+
H+

Synonyms

AaBDH, ADH2, BDH, BDH1, BDH2, BDH3, bicyclic monoterpenol dehydrogenase, borneol dehydrogenase, borneol-type dehydrogenase 1, EC 1.1.1.182, TCK1_3449, THL1_4180

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.198 (+)-borneol dehydrogenase

Purification

Purification on EC 1.1.1.198 - (+)-borneol dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl Sepharose column chromatography and Q-Sepharose column chromatography
ammonium sulfate saturation, phenyl Sepharose column chromatography, and Q Sepharose column chromatography
His-Trap column chromatography
-
HisTrap column chromatography
Pseudomonas sp. ATCC 17453
-
near homogeneity, chromatography techniques, affinity chromatography
-
Ni-NTA affinity column chromatography
partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns
-
phenyl-Sepharose column chromatography, Q-Sepharose column chromatography and MonoQ column chromatography
-
production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium
recombinant enzyme refolded from inclusion bodies after expression in Escherichia coli strain BL21 by anion exchange chromatography and dialysis for refolding, native enzyme from strain TCU-HL1 by hydrophobic interaction and anion exchange chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration