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2.2
(R)-2-butanol
-
pH 7.5, 37°C
4.5
(S)-2-butanol
-
pH 7.5, 37°C
0.07 - 0.117
1,2-naphthoquinone
0.16 - 1.13
1,4-benzoquinone
0.0073 - 0.54
1,4-Naphthoquinone
0.156 - 0.469
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone
0.105 - 0.116
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone
0.04
1-phenyl-1,2-propanedione
-
pH 6.0, recombinant enzyme
0.004 - 0.009
1-phenylisatin
0.036 - 0.099
16-ketoestrone
0.3
2,2,2-trifluoroacetophenone
pH 9.0, 70°C, recombinant enzyme
18
2,3-Butanediol
-
pH 7.5, 37°C
0.22
2,3-heptanedione
-
pH 6.0
0.47
2,3-hexandione
-
pH 6.0, recombinant enzyme
0.06 - 0.62
2,3-Hexanedione
3.9 - 33
2,3-Pentanedione
0.93 - 1.7
2-benzoylpyridine
3.5
2-butanol
-
pH 7.5, 37°C
9.5
2-butanone
-
pH 7.5, 37°C
0.59 - 10.27
2-hydroxyacetophenone
0.57
2-methyl-1,4-benzoquinone
-
-
0.8
2-nitrobenzaldehyde
-
-
14
2-Pentanone
-
pH 7.5, 37°C
3
2-propanol
-
pH 7.5, 37°C
0.33 - 0.76
3-benzoylpyridine
0.033
3-glutathionyl-4-hydroxynonanal
-
pH 8.4, 37°C
0.0238
3-glutathionyl-hexanal
pH 8.4, 25°C
0.0065
3-glutathionyl-nonanal
pH 8.4, 25°C
0.0207
3-glutathionyl-propanal
pH 8.4, 25°C
5.85 - 7.84
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
1.55
4-acetylpyridine
-
-
0.000000053 - 3.3
4-benzoylpyridine
0.38 - 0.65
4-formylbenzoic acid
-
Km depending on enzyme form
0.044 - 0.09
4-hexanoylpyridine
0.004 - 2.5
4-Nitroacetophenone
0.12 - 2
4-nitrobenzaldehyde
0.345
4-oxonon-2-enal
-
pH 7.4, 37°C, recombinant enzyme
0.05 - 0.1
5alpha-androstan-17beta-ol-3-one
0.15
5alpha-androstane-3,17-dione
-
form CR2
0.002 - 0.078
9,10-phenanthrenequinone
0.002
9,10-phenanthrenquinone
-
-
0.003
9,10-phenanthroline
-
pH 5.5, 37°C
0.0046
9,10-phenantrenequinone
at pH 8.4 and 37°C
-
0.087
9-cis-retinal
-
pH 7.4
0.02
acenaphthenequinone
-
pH 6.0
0.92
acetohexamide
-
pH 6.5, 30°C
16
acetoin
-
pH 7.5, 37°C
0.083
Adrenochrome
-
pH 6.0, 37°C
0.009
all-trans-retinal
-
pH 7.4
0.086
all-trans-retinol
-
pH 7.4
1.44
befunolol
-
pH 6.5, 30°C
0.031 - 0.33
Benzalacetone
0.13
benzil
-
pH 6.0, recombinant enzyme
0.008
benzoylformic acid ethyl ester
-
pH 6.0
0.084
butyrophenone
-
pH 6.0, 37°C
0.12 - 250
crotonaldehyde
6.5
cyclohexanone
-
pH 7.5, 37°C
4.5
Cyclopentanol
-
pH 7.5, 37°C
4.2
Cyclopentanone
-
pH 7.5, 37°C
0.041 - 0.335
doxorubicin
0.001
estrone
pH 6.5, 25°C
4.1
ethyl 2-chloro-3-oxobutanoate
-
-
4.9
ethyl 2-methylacetoacetate
pH 9.0, 70°C, recombinant enzyme
0.319
ethyl 2-oxo-4-phenylbutanoate
-
pH 7.0, 30°C
6
ethyl 2-oxo-4-phenylbutyrate
pH 9.0, 70°C, recombinant enzyme
61
ethyl 2-oxobutanoate
-
-
0.0026 - 4.6
ethyl 4-chloro-3-oxobutanoate
1.85 - 1.855
ethyl 4-chloroacetoacetate
0.329
glutathione-4-oxonon-2-enal
-
pH 7.4, 37°C, recombinant enzyme
0.0065
glutathionyl-nonanal
at pH 8.4 and 37°C
-
0.018 - 0.078
heptanophenone
0.011 - 0.037
hexanophenone
0.02
hexaphenone
-
pH 6.0, 25°C, recombinant enzyme
10
ketopantoyl lactone
-
-
0.52
loxoprofen
-
pH 6.5, 30°C
0.000000067 - 0.527
menadione
39
methyl 2-chloro-3-oxobutanoate
-
-
10
methyl 4-chloro-3-oxobutanoate
-
-
0.43 - 1.6
methyl glyoxal
0.012 - 0.057
methyl-1,4-benzoquinone
2.2
methylglyoxal
pH 7.0, 25°C
0.029 - 0.17
n-butyrophenone
0.021
NADP+
-
pH 7.5, 37°C
0.031
nonanophenone
-
pH 6.0, recombinant enzyme
0.176 - 0.18
p-toluquinone
0.4
propanone
-
pH 7.5, 37°C
0.249 - 0.89
propiophenone
0.14 - 0.21
Prostaglandin B2
-
Km depending on enzyme form
0.45
prostaglandin E1
-
enzyme form CR8
0.03 - 0.32
prostaglandin E2
0.09
pyridin-4-yl hexanoate
-
pH 6.0, 25°C, recombinant enzyme
0.015 - 0.13
pyridine 3-aldehyde
0.002
pyridine 4-aldehyde
-
cofactor NADPH
1.8 - 11.4
Pyridine-3-aldehyde
0.5 - 1.4
Pyridine-4-aldehyde
0.49 - 2
pyridine-4-carboxaldehyde
0.028
S-1-phenyl-1-butanol
-
pH 7.4
0.028 - 2.93
S-nitrosoglutathione
1.3
t-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate
-
at pH 7.0 and 25°C
-
0.04
testosterone
pH 6.5, 25°C
0.015 - 0.017
ubiquinone-1
-
Km depending on enzyme form
0.01 - 0.052
valerophenone
additional information
additional information
-
0.07
1,2-naphthoquinone
wild-type, pH 7.4, 25°C
0.07
1,2-naphthoquinone
wild-type, pH 7.4, 37°C
0.117
1,2-naphthoquinone
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.117
1,2-naphthoquinone
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.16
1,4-benzoquinone
-
pH 6.0, 25°C, recombinant enzyme
1.13
1,4-benzoquinone
-
-
0.0073
1,4-Naphthoquinone
wild-type, pH 7.4, 25°C
0.0073
1,4-Naphthoquinone
wild-type, pH 7.4, 37°C
0.032
1,4-Naphthoquinone
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.032
1,4-Naphthoquinone
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.09
1,4-Naphthoquinone
-
pH 6.0, 25°C, recombinant enzyme
0.54
1,4-Naphthoquinone
-
-
0.156
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone
pH 7.4, 37°C
0.469
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone
pH 7.4, 37°C
0.105
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone
pH 7.4, 37°C
0.116
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone
pH 7.4, 37°C
0.004
1-phenylisatin
-
pH 6.0, recombinant enzyme
0.009
1-phenylisatin
-
pH 6.0
0.036
16-ketoestrone
-
pH 7.4
0.086
16-ketoestrone
-
pH 6.0, recombinant enzyme
0.099
16-ketoestrone
-
pH 6.0
0.06
2,3-Hexanedione
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.06
2,3-Hexanedione
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.159
2,3-Hexanedione
wild-type, pH 7.4, 25°C
0.159
2,3-Hexanedione
wild-type, pH 7.4, 37°C
0.62
2,3-Hexanedione
-
pH 6.0
3.9
2,3-Pentanedione
-
pH 6.0
5.7
2,3-Pentanedione
pH 7.0, 25°C
10.8
2,3-Pentanedione
pH 7.0, 25°C
0.93
2-benzoylpyridine
-
pH 6.0, 25°C, recombinant enzyme
1.7
2-benzoylpyridine
-
-
0.59
2-hydroxyacetophenone
wild-type, NADPH as cofactor
0.59
2-hydroxyacetophenone
wild-type, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
0.63
2-hydroxyacetophenone
mutant DELTA31, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
0.68
2-hydroxyacetophenone
mutant S172T, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
0.71
2-hydroxyacetophenone
mutant V270D, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
0.76
2-hydroxyacetophenone
mutant S67D/H68D, NADH as cofactor
0.76
2-hydroxyacetophenone
mutant S67D/H68D, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.22
2-hydroxyacetophenone
mutant P69D, NADH as cofactor
1.26
2-hydroxyacetophenone
mutant S67D, NADH as cofactor
1.35
2-hydroxyacetophenone
mutant H68D, NADH as cofactor
1.42
2-hydroxyacetophenone
mutant S67D/P69D, NADH as cofactor
1.46
2-hydroxyacetophenone
mutant H68D/P69D, NADH as cofactor
1.51
2-hydroxyacetophenone
mutant P69D, NADPH as cofactor
1.54
2-hydroxyacetophenone
mutant S67D, NADPH as cofactor
1.55
2-hydroxyacetophenone
mutant S67D/P69D, NADPH as cofactor
1.58
2-hydroxyacetophenone
mutant H68D, NADPH as cofactor
1.61
2-hydroxyacetophenone
mutant H68D/P69D, NADPH as cofactor
7.89
2-hydroxyacetophenone
wild-type, NADH as cofactor
7.89
2-hydroxyacetophenone
wild-type, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
8.03
2-hydroxyacetophenone
mutant DELTA31, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
8.45
2-hydroxyacetophenone
mutant V270D, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
8.67
2-hydroxyacetophenone
mutant S172T, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
10.27
2-hydroxyacetophenone
mutant S67D/H68D, NADPH as cofactor
10.27
2-hydroxyacetophenone
mutant S67D/H68D, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
0.33
3-benzoylpyridine
-
-
0.63
3-benzoylpyridine
-
-
0.76
3-benzoylpyridine
-
pH 6.0, 25°C, recombinant enzyme
0.76
3-benzoylpyridine
-
pH 6.0, recombinant enzyme
5.85
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
7.84
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
wild-type, pH 7.4, 37°C
0.000000053
4-benzoylpyridine
-
wild-type
0.000000058
4-benzoylpyridine
-
mutant P230W
0.00000054
4-benzoylpyridine
-
mutant W230P
0.00000062
4-benzoylpyridine
-
wild-type
0.0000015
4-benzoylpyridine
wild-type
0.0000018
4-benzoylpyridine
mutant W230P
0.016
4-benzoylpyridine
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.016
4-benzoylpyridine
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.043
4-benzoylpyridine
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.24
4-benzoylpyridine
-
cofactor NADPH
0.25
4-benzoylpyridine
wild-type, pH 7.4, 25°C
0.25
4-benzoylpyridine
wild-type, pH 7.4, 37°C
0.29
4-benzoylpyridine
-
pH 6.0, 25°C, recombinant enzyme
0.29
4-benzoylpyridine
-
pH 6.0, recombinant enzyme
0.3
4-benzoylpyridine
pH 6.5, 25°C
0.31 - 0.45
4-benzoylpyridine
-
Km depending on enzyme form
0.386
4-benzoylpyridine
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.4
4-benzoylpyridine
-
pH 6.0
0.435
4-benzoylpyridine
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
3.3
4-benzoylpyridine
-
cofactor NADH
0.044
4-hexanoylpyridine
-
pH 6.0
0.09
4-hexanoylpyridine
-
pH 6.0, recombinant enzyme
0.004
4-Nitroacetophenone
-
cofactor NADPH
0.59
4-Nitroacetophenone
-
-
1.2
4-Nitroacetophenone
-
pH 6.0, 25°C, recombinant enzyme
1.3 - 2.5
4-Nitroacetophenone
-
Km depending on enzyme form
0.12
4-nitrobenzaldehyde
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.13
4-nitrobenzaldehyde
pH 6.5, 25°C
0.2
4-nitrobenzaldehyde
-
pH 6.0, 25°C, recombinant enzyme
0.338
4-nitrobenzaldehyde
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.354
4-nitrobenzaldehyde
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.44
4-nitrobenzaldehyde
pH 6.5, 25°C
0.45
4-nitrobenzaldehyde
-
-
0.64
4-nitrobenzaldehyde
-
recombinant protein purified from E. coli
1.1
4-nitrobenzaldehyde
-
-
1.3 - 1.6
4-nitrobenzaldehyde
-
Km depending on enzyme form
1.6 - 2
4-nitrobenzaldehyde
-
Km depending on enzyme form
0.05
5alpha-androstan-17beta-ol-3-one
-
form CR2
0.1
5alpha-androstan-17beta-ol-3-one
-
-
0.002
9,10-phenanthrenequinone
-
pH 6.0
0.002
9,10-phenanthrenequinone
pH 6.5, 25°C
0.002
9,10-phenanthrenequinone
-
pH 6.0, recombinant enzyme
0.0043
9,10-phenanthrenequinone
residues 142-143, 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1
0.0089
9,10-phenanthrenequinone
residues 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1
0.0092
9,10-phenanthrenequinone
residues 97-98, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1
0.0128
9,10-phenanthrenequinone
mutant C227S, pH 7.4, temperature not specified in the publication
0.0145
9,10-phenanthrenequinone
pH 6.0, 50°C
0.018
9,10-phenanthrenequinone
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.018
9,10-phenanthrenequinone
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.032
9,10-phenanthrenequinone
wild-type, pH 7.4, 25°C
0.032
9,10-phenanthrenequinone
wild-type, pH 7.4, 37°C
0.0354
9,10-phenanthrenequinone
wild-type CBR1
0.0354
9,10-phenanthrenequinone
wild-type, pH 7.4, temperature not specified in the publication
0.0393
9,10-phenanthrenequinone
residues 142-143, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1
0.0564
9,10-phenanthrenequinone
residues 230, 236244 of CBR3 exchanged to the corresponding amino acids of CBR1
0.078
9,10-phenanthrenequinone
wild-type CBR3 and mutant V244M. Residues 236-244, 262-277, 236-244/262-277 or 230 of CBR3 exchanged to the corresponding amino acids of CBR1
0.039
acetaldehyde
-
cofactor NADPH
5.1
acetaldehyde
-
cofactor NADH
0.05
acetone
pH 6.5, 25°C
0.091
acetone
-
cofactor NADPH
3.1
acetone
-
cofactor NADH
0.031
Benzalacetone
-
cofactor NADPH
0.33
Benzalacetone
-
cofactor NADH
0.12
crotonaldehyde
-
cofactor NADPH
250
crotonaldehyde
-
cofactor NADH
0.043
daunorubicin
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.051
daunorubicin
wild-type
0.051
daunorubicin
single nucleotide polymorphism V88I mutation
0.067
daunorubicin
wild-type, pH 7.4, 37°C
0.0852
daunorubicin
pH 7.4, 37°C
0.089
daunorubicin
single nucleotide polymorphism P131S mutation
0.104
daunorubicin
J9P7P2
mutant D218V, pH 7.4, 37°C
0.13
daunorubicin
-
enzyme form CR8
0.14
daunorubicin
-
mutant V88I, 37°C, pH 7.4
0.173
daunorubicin
-
wild-type, 37°C, pH 7.4
0.188
daunorubicin
J9P7P2
wild-type, pH 7.4, 37°C
3
daunorubicin
-
pH 6.5, 30°C
0.0136
diacetyl
-
pH 7.4, 30°C
3
diacetyl
-
pH 7.5, 37°C
4.5
diacetyl
-
pH 6.0, recombinant enzyme
0.041
doxorubicin
isoform Cbr3, at pH 7.0 and 37°C
0.13
doxorubicin
isoform Cbr1, at pH 7.0 and 37°C
0.132
doxorubicin
in stomach
0.134
doxorubicin
in kidney
0.14
doxorubicin
in colon
0.163
doxorubicin
in liver
0.167
doxorubicin
purified enzyme
0.231
doxorubicin
in lung
0.239
doxorubicin
in heart
0.244
doxorubicin
in skeletal muscle
0.287
doxorubicin
wild-type
0.325
doxorubicin
single nucleotide polymorphism P131S mutation
0.335
doxorubicin
single nucleotide polymorphism V88I mutation
0.0026
ethyl 4-chloro-3-oxobutanoate
-
pH 6.5, 30°C, recombinant mutant S1M4
0.003
ethyl 4-chloro-3-oxobutanoate
-
pH 6.5, 30°C, recombinant wild-type enzyme
0.0039
ethyl 4-chloro-3-oxobutanoate
-
pH 6.5, 30°C, recombinant mutant S1M1
0.7
ethyl 4-chloro-3-oxobutanoate
pH 6.0, 50°C
4.6
ethyl 4-chloro-3-oxobutanoate
-
-
1.85
ethyl 4-chloroacetoacetate
-
at pH 7.0 and 37°C
1.855
ethyl 4-chloroacetoacetate
-
at pH 6.5 and 30°C
0.018
heptanophenone
-
pH 6.0
0.028
heptanophenone
-
pH 6.0, recombinant enzyme
0.078
heptanophenone
-
pH 6.0, 37°C
0.011
hexanophenone
-
pH 6.0
0.021
hexanophenone
-
pH 6.0, recombinant enzyme
0.037
hexanophenone
-
pH 6.0, 37°C
0.0008
isatin
wild-type, pH 7.4, 37°C
0.00183
isatin
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.0026
isatin
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.0078
isatin
wild-type CBR1
0.008
isatin
wild-type, pH 7.4, 25°C
0.017
isatin
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.017
isatin
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.0225
isatin
residues 142-143, 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1
0.03
isatin
-
pH 6.0, recombinant enzyme
0.0315
isatin
residues 142-143, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1
0.0327
isatin
residues 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1
0.0471
isatin
residues 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1
0.055
isatin
residues 97-98, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1
0.078
isatin
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
1.6
isatin
residues 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1
4
isatin
wild-type CBR3 and mutant V244M. Residues 262-277, 236-244/262-277 or 230 of CBR3 exchanged to the corresponding amino acids of CBR1
0.000000067
menadione
-
wild-type
0.000000079
menadione
-
mutant P230W
0.000000089
menadione
-
wild-type
0.000000097
menadione
-
mutant W230P
0.0000003
menadione
wild-type and mutant W230P
0.009
menadione
-
cofactor NADPH
0.014
menadione
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.014
menadione
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.018
menadione
wild-type, pH 7.4, 25°C
0.018
menadione
wild-type, pH 7.4, 37°C
0.018
menadione
single nucleotide polymorphism V88I mutation
0.0199
menadione
pH 7.4, 37°C
0.0229
menadione
-
pH 7.4, 37°C, recombinant isozyme CBR M244
0.024
menadione
wild-type
0.0246
menadione
-
pH 7.4, 37°C, recombinant isozyme CBR V244
0.025
menadione
-
pH 7.4, 37°C, recombinant enzyme
0.04
menadione
pH 6.5, 25°C
0.042
menadione
-
wild-type, 37°C, pH 7.4
0.043
menadione
pH 6.5, 25°C
0.045
menadione
single nucleotide polymorphism P131S mutation
0.045 - 0.06
menadione
-
Km depending on enzyme form
0.05 - 0.07
menadione
-
Km depending on enzyme form
0.055
menadione
-
mutant V88I, 37°C, pH 7.4
0.076
menadione
pH 7.4, 37°C
0.118
menadione
J9P7P2
mutant D218V, pH 7.4, 37°C
0.29
menadione
-
pH 6.0, 25°C, recombinant enzyme
0.29
menadione
-
pH 6.0, recombinant enzyme
0.527
menadione
J9P7P2
wild-type, pH 7.4, 37°C
0.43
methyl glyoxal
pH 6.0, 25°C
1.6
methyl glyoxal
pH 6.0, 25°C
0.012
methyl-1,4-benzoquinone
-
cofactor NADPH
0.057
methyl-1,4-benzoquinone
-
cofactor NADH
0.029
n-butyrophenone
-
pH 6.0
0.17
n-butyrophenone
-
pH 6.0, 25°C, recombinant enzyme
0.17
n-butyrophenone
-
pH 6.0, recombinant enzyme
0.062
NADH
-
pH 6.5, 30°C, recombinant mutant S1M4
0.16
NADH
-
pH 6.5, 30°C, recombinant mutant S1M1
0.3
NADH
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.43
NADH
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.76
NADH
mutant S67D/H68D, pH 7.5, 35°C
7.89
NADH
wild-type, pH 7.5, 35°C
8.03
NADH
deletion mutant lacking 31 N-terminal amino acids, pH 7.5, 35°C
8.45
NADH
mutant V270D, pH 7.5, 35°C
8.67
NADH
mutant S172T, pH 7.5, 35°C
0.00214
NADPH
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.0022
NADPH
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.004 - 0.01
NADPH
-
+ 4-nitrobenzaldehyde, Km depending on enzyme form
0.0045
NADPH
pH 8.4, 25°C
0.0167
NADPH
-
pH 6.5, 30°C, recombinant wild-type enzyme
0.0218
NADPH
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.025
NADPH
-
pH 7.5, 37°C
0.0539
NADPH
-
at pH 6.5 and 30°C
0.0612
NADPH
pH 7.0, 25°C
0.074
NADPH
isoform Cbr3, at pH 7.0 and 37°C
0.085
NADPH
-
pH 7.4, 37°C, recombinant isozyme CBR M244
0.0905
NADPH
-
pH 7.4, 37°C, recombinant isozyme CBR V244
0.099
NADPH
isoform Cbr1, at pH 7.0 and 37°C
0.247
NADPH
-
wild-type, 37°C, pH 7.4
0.343
NADPH
-
mutant V88I, 37°C, pH 7.4
0.59
NADPH
wild-type, pH 7.5, 35°C
0.63
NADPH
deletion mutant lacking 31 N-terminal amino acids, pH 7.5, 35°C
0.68
NADPH
mutant S172T, pH 7.5, 35°C
0.71
NADPH
mutant V270D, pH 7.5, 35°C
10.27
NADPH
mutant S67D/H68D, pH 7.5, 35°C
0.034
oracin
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.096
oracin
wild-type, pH 7.4, 37°C
0.176
p-toluquinone
pH 6.0, 50°C
0.18
p-toluquinone
pH 6.5, 25°C
1
Phenylglyoxal
-
-
4 - 5
Phenylglyoxal
-
Km depending on enzyme form
4.8
Phenylglyoxal
pH 7.0, 25°C
0.249
propiophenone
-
pH 6.0, 37°C
0.89
propiophenone
-
pH 6.0, 25°C, recombinant enzyme
0.89
propiophenone
-
pH 6.0, recombinant enzyme
0.03
prostaglandin E2
pH 6.5, 25°C
0.16
prostaglandin E2
-
-
0.24
prostaglandin E2
-
form CR2
0.309
prostaglandin E2
-
wild-type, 37°C, pH 7.4
0.32
prostaglandin E2
-
mutant V88I, 37°C, pH 7.4
0.015
pyridine 3-aldehyde
-
cofactor NADPH
0.13
pyridine 3-aldehyde
-
cosubstrate NADH
1.8
Pyridine-3-aldehyde
-
-
11.4
Pyridine-3-aldehyde
-
pH 6.0, 25°C, recombinant enzyme
0.5
Pyridine-4-aldehyde
-
cofactor NADH
0.9
Pyridine-4-aldehyde
-
-
1.4
Pyridine-4-aldehyde
-
pH 6.0, 25°C, recombinant enzyme
1.4
Pyridine-4-aldehyde
-
pH 6.0, recombinant enzyme
0.49 - 2
pyridine-4-carboxaldehyde
-
Km depending on enzyme form
1.4
pyridine-4-carboxaldehyde
-
-
0.028
S-nitrosoglutathione
wild-type, pH 7.4, temperature not specified in the publication
0.81
S-nitrosoglutathione
mutant C227S, pH 7.4, temperature not specified in the publication
0.832
S-nitrosoglutathione
mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication
1.09
S-nitrosoglutathione
mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication
2.93
S-nitrosoglutathione
mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication
0.01
valerophenone
-
pH 6.0
0.048
valerophenone
-
pH 6.0, 37°C
0.05
valerophenone
-
pH 6.0, 25°C, recombinant enzyme
0.052
valerophenone
-
pH 6.0, recombinant enzyme
additional information
additional information
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-
-
additional information
additional information
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-
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additional information
additional information
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-
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additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
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-
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additional information
additional information
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kinetics
-
additional information
additional information
-
overview
-
additional information
additional information
-
overview
-
additional information
additional information
-
overview
-
additional information
additional information
-
pharmacokinetics of acetohexamide in male Wistar-Imamichi, showing very low enzyme amount, and Sprague-Dawley rats, overview
-
additional information
additional information
-
reaction and cofactor binding kinetics of wild-type and mutant enzymes, thermodynamics, overview
-
additional information
additional information
recombinant enzyme, steady-state kinetics, molecular modeling and substrate docking studies
-
additional information
additional information
-
the glucocorticoid and xenobiotic reductase activities show cooperative kinetics
-
1.1.1.184 carbonyl reductase (NADPH)
results (
results (
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