1.1.1.179: D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming)
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For detailed information about D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming), go to the full flat file.
Reaction
Synonyms
D-xylose (nicotinamide adenine dinucleotide phosphate) dehydrogenase, D-xylose 1-dehydrogenase (NADP), D-xylose dehydrogenase, D-xylose-NADP dehydrogenase, D-xylose:NADP+ oxidoreductase, NADP+-dependent D-xylose dehydrogenase, NADP+:D-xylose dehydrogenase, XDH, xyd1
ECTree
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Cloned
Cloned on EC 1.1.1.179 - D-xylose 1-dehydrogenase (NADP+, D-xylono-1,5-lactone-forming)
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cDNA subcloned in expression vector pRset, overexpression in Escherichia coli
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cloned into pET19b, resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain
gene xyd1, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Saccharomyces cerevisiae
A8BT09
wild-type protein and mutants are expressed in Escherichia coli BL21 (DE3)