1.1.1.169: 2-dehydropantoate 2-reductase
This is an abbreviated version!
For detailed information about 2-dehydropantoate 2-reductase, go to the full flat file.
Word Map on EC 1.1.1.169
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1.1.1.169
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pantothenate
-
nadph-dependent
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pane
-
hydroxymethyltransferase
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ketopantoyl
-
2'-phosphate
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fragment-based
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d-2-hydroxyacid
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parapsilosis
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kodakarensis
- 1.1.1.169
- pantothenate
-
nadph-dependent
-
pane
- hydroxymethyltransferase
-
ketopantoyl
- 2'-phosphate
-
fragment-based
- d-2-hydroxyacid
-
parapsilosis
- kodakarensis
Reaction
Synonyms
2-dehydropantoate 2-reductase, 2-ketopantoate reductase, 2-ketopantoic acid reductase, 2-oxopantoate reductase, conjugated polyketone reductase, CPR, ilvC, ketopantoate reductase, ketopantoic acid reductase, KPA reductase, KPR, More, PanE, panG, Tk-KPR, TK1968, TK1968 protein
ECTree
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Purification
Purification on EC 1.1.1.169 - 2-dehydropantoate 2-reductase
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gel chromatography
native wild-type enzyme by anion exchange and adsorption chromatography, and gel filtration, recombinant His6-tagged enzyme by nickel affinity chromatography
on nickel-nitrilotriacetic acid resin and by gel filtration, more than 98% pure
recombinant C-terminally His-tagged wild-type and mutant enzymes
recombinant enzyme from Escherichia coli by heat treatment, anion-exchange chromatography, and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta2 (DE3) pLysS by nickel affinity chromatography, avidin affinity chromatography, and dialysis
recombinant wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer from Escherichia coli by affinity chromatography