Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.79
(+)-11-dihydrooracin
-
37°C, pH 10.8-11.0
-
0.075
1,2-dehydrocortisol
-
-
0.0002 - 0.0073
11-Dehydrocorticosterone
0.0014 - 0.027
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
0.000034 - 0.0195
11beta,21-dihydroxypregn-4-en-3,20-dione
0.000685
11beta-hydroxyandrostenedione
isoform 11betaHSD2, pH and temperature not specified in the publication
0.000023 - 0.0169
11beta-hydroxyprogesterone
0.000056 - 0.000147
11beta-hydroxytestosterone
0.204
16alpha-methyl-1,2-dehydrocortisol
-
-
0.0487
16alpha-Methylcortisol
-
-
0.0038 - 0.0095
17,21-dihydroxy-pregn-4-ene-3,11,20-trione
0.02
2,3-benzoylphenyl-propanoic acid
-
microsomal fraction from transformed Pichia pastoris strain with human enzyme
0.00144 - 0.00283
21-hydroxy-pregn-4-en-3,11,20-trione
0.581 - 0.961
5,5-dimethyl-3-(3-fluorphenyl)-4-(4-methylsulphonyl)phenyl-2-(5H-furanone)-lactol
0.0012
5alpha-androstane-3beta,7alpha,17beta-triol
-
pH 7.4, 28°C
0.021
5alpha-androstane-3beta,7beta,17beta-triol
-
pH 7.4, 28°C
0.000039
7-keto,27-hydroxycholesterol
isoform 11beta-HSD1, at pH 7.4, temperature not specified in the publication
0.0051 - 0.0068
7-oxo-5alpha-androstane-3beta,17beta-diol
1.269
7-oxo-cholesterol
-
recombinant enzyme, at pH 7.4 and 37°C
0.00038 - 0.00049
7-oxocholesterol
0.00049
7alpha-hydroxycholesterol
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.000029 - 0.000556
7beta,25-dihydroxycholesterol
0.000056
7beta,27-dihydroxycholesterol
isoform 11beta-HSD2, at pH 7.4, temperature not specified in the publication
0.00073 - 3.5
7beta-hydroxycholesterol
0.0003 - 0.043
corticosterone
0.00028 - 0.193
cortisone
0.02
dehydrocorticosterone
-
37°C, pH 7.4, Hill coefficient of 2.7
0.37
metapyrone
-
microsomal fraction from transformed Pichia pastoris strain with human enzyme
0.0000063 - 0.00427
NADP+
additional information
additional information
-
0.0002
11-Dehydrocorticosterone
-
recombinant enzyme, at pH 7.4 and 37°C
0.00025
11-Dehydrocorticosterone
-
pH 7.4, 37°C
0.00031
11-Dehydrocorticosterone
-
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.00031
11-Dehydrocorticosterone
-
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.0004
11-Dehydrocorticosterone
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.0013
11-Dehydrocorticosterone
-
native enzyme, at pH 7.4 and 37°C
0.0025
11-Dehydrocorticosterone
-
37°C, pH 7.4, recombinant 11beta-HSD1
0.0073
11-Dehydrocorticosterone
-
37°C, pH 7.4
0.0014
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
i.e. cortisol
0.0014
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
recombinant catalytic domain
0.0092
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
i.e. cortisol
0.0092
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
microsomal fraction from transformed Pichia pastoris strain with human enzyme
0.0102
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
i.e. cortisol
0.0102
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
purified recombinant enzyme
0.0173
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
i.e. cortisol
0.0249
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
i.e. cortisol
0.027
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione
-
-
0.000034
11beta,21-dihydroxypregn-4-en-3,20-dione
-
pH and temperature not specified in the publication
0.00108
11beta,21-dihydroxypregn-4-en-3,20-dione
-
-
0.00108
11beta,21-dihydroxypregn-4-en-3,20-dione
-
i.e. cortisol,temperature not specified in the publication, pH 9
0.0011
11beta,21-dihydroxypregn-4-en-3,20-dione
-
at pH 7.0
0.00183
11beta,21-dihydroxypregn-4-en-3,20-dione
-
-
0.002
11beta,21-dihydroxypregn-4-en-3,20-dione
-
isoform 11beta-HSD1
0.00214
11beta,21-dihydroxypregn-4-en-3,20-dione
-
at pH 8.5
0.0027
11beta,21-dihydroxypregn-4-en-3,20-dione
-
-
0.0195
11beta,21-dihydroxypregn-4-en-3,20-dione
-
-
0.000023
11beta-hydroxyprogesterone
isoform 11betaHSD2, pH and temperature not specified in the publication
0.0169
11beta-hydroxyprogesterone
-
-
0.000056
11beta-hydroxytestosterone
-
pH 7.4, 37°C, microsomal assay
0.000147
11beta-hydroxytestosterone
isoform 11betaHSD2, pH and temperature not specified in the publication
0.0038
17,21-dihydroxy-pregn-4-ene-3,11,20-trione
-
i.e. cortisone
0.0038
17,21-dihydroxy-pregn-4-ene-3,11,20-trione
-
microsomal fraction from transformed Pichia pastoris strain with human enzyme
0.00875
17,21-dihydroxy-pregn-4-ene-3,11,20-trione
-
i.e. cortisone
0.00875
17,21-dihydroxy-pregn-4-ene-3,11,20-trione
-
i.e. cortisone, temperature not specified in the publication, pH 9
0.0095
17,21-dihydroxy-pregn-4-ene-3,11,20-trione
-
i.e. cortisone
0.0095
17,21-dihydroxy-pregn-4-ene-3,11,20-trione
-
recombinant catalytic domain
0.00144
21-hydroxy-pregn-4-en-3,11,20-trione
-
at pH 7.0
0.00156
21-hydroxy-pregn-4-en-3,11,20-trione
-
-
0.00156
21-hydroxy-pregn-4-en-3,11,20-trione
-
temperature not specified in the publication, pH 9
0.00283
21-hydroxy-pregn-4-en-3,11,20-trione
-
at pH 8.5
0.581
5,5-dimethyl-3-(3-fluorphenyl)-4-(4-methylsulphonyl)phenyl-2-(5H-furanone)-lactol
-
microsomal fraction from transformed Pichia pastoris strain with human enzyme
0.961
5,5-dimethyl-3-(3-fluorphenyl)-4-(4-methylsulphonyl)phenyl-2-(5H-furanone)-lactol
-
purified recombinant enzyme
0.0051
7-oxo-5alpha-androstane-3beta,17beta-diol
-
product 5alpha-androstane-3beta,7alpha,17beta-triol, pH 7.4, 28°C
0.0068
7-oxo-5alpha-androstane-3beta,17beta-diol
-
product 5alpha-androstane-3beta,7beta,17beta-triol, pH 7.4, 28°C
0.00038
7-oxocholesterol
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.00042
7-oxocholesterol
-
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.00049
7-oxocholesterol
-
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.000029
7beta,25-dihydroxycholesterol
recombinant isoform 11beta-HSD1, at pH 7.4 and 37°C
0.000556
7beta,25-dihydroxycholesterol
recombinant isoform 11beta-HSD2, at pH 7.4 and 37°C
0.00073
7beta-hydroxycholesterol
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.00074
7beta-hydroxycholesterol
-
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.00075
7beta-hydroxycholesterol
-
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.3176
7beta-hydroxycholesterol
-
recombinant enzyme, at pH 7.4 and 37°C
3.5
7beta-hydroxycholesterol
-
native enzyme, at pH 7.4 and 37°C
0.0003
corticosterone
-
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.0012
corticosterone
-
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.00178
corticosterone
-
recombinant enzyme, at pH 7.4 and 37°C
0.003
corticosterone
-
37°C, pH 7.4, recombinant 11beta-HSD1
0.0032
corticosterone
37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1
0.0042
corticosterone
-
native enzyme, at pH 7.4 and 37°C
0.043
corticosterone
-
37°C, pH 7.4, Hill coefficient of 0.7
0.000237
cortisol
-
enzyme from caput epididymidis, cosubstrate NADP+, 37°C, pH not specified in the publication
0.000254
cortisol
-
enzyme from testis, cosubstrate NADP+, 37°C, pH not specified in the publication
0.00034
cortisol
-
37°C, pH 7.4, oxidation of cortisol by lysates of HEK-293 cells transfected with 11beta-HSD1
0.000433
cortisol
-
enzyme from bulbourethral gland, cosubstrate NADP+, 37°C, pH not specified in the publication
0.0008
cortisol
-
37°C, pH 7.4, oxidation of cortisol in intact HEK-293 cells transfected with 11beta-HSD1
0.0017
cortisol
-
37°C, pH 7.4, recombinant 11beta-HSD1
0.002
cortisol
-
kidney enzyme, pH 7.4, 37°C
0.003
cortisol
-
37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli
0.003
cortisol
-
37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli
0.0065
cortisol
-
pH 7.4, 28°C
0.0096
cortisol
-
pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1
0.041
cortisol
-
37°C, pH 7.4, Hill coefficient of 1.0, no cooperative kinetic
0.042
cortisol
-
pH 7.5, 22°C, recombinant wild-type isozyme 11beta-HSD1 oxidized with K3Fe(CN)6
0.1
cortisol
-
37°C, pH 7.4, V149R mutant 11beta-HSD1, V149E mutant 11beta-HSD1, and N-terminal 15 amino acid truncated V149R and V149E mutant 11beta-HSD1
0.00028
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1
0.0003
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1
0.00034
cortisone
-
37°C, pH 7.4, reduction of cortisone by lysates of HEK-293 cells transfected with 11beta-HSD1
0.0006
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1
0.0006
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli
0.0008
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli
0.0008
cortisone
-
37°C, pH 7.4, reduction of cortisone in intact HEK-293 cells transfected with 11beta-HSD1
0.0008
cortisone
-
kidney enzyme, pH 7.4, 37°C
0.002
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Pichia pastoris
0.002
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Pichia pastoris
0.0044
cortisone
-
pH 7.4, 28°C
0.008
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1 measured in extracts of COS cells transfected with 11beta-HSD1
0.009
cortisone
-
37°C, pH 7.4, recombinant 11beta-HSD1 measured in extracts of COS cells transfected with 11beta-HSD1
0.01
cortisone
-
liver enzyme, pH 7.4, 37°C
0.014
cortisone
-
37°C, pH 7.4, Hill coefficient of 1.6
0.1
cortisone
-
37°C, pH 7.4, V149R mutant 11beta-HSD1, V149E mutant 11beta-HSD1, and N-terminal 15 amino acid truncated V149R and V149E mutant 11beta-HSD1
0.193
cortisone
at pH 7.5 and 37°C
0.0000102
NAD+
-
isoform 11beta-HSD2, kidney homogenate
0.0000063
NADP+
-
reduction of corticosterone, pH and temperature not specified in the publication
0.000196
NADP+
-
reduction of corticosterone, ordered sequential mechanism
0.00042
NADP+
-
isoform 11beta-HSD1, kidney homogenate
0.00042
NADP+
-
kidney homogenate, pH and temperature not specified in the publication
0.00427
NADP+
-
temperature not specified in the publication, pH 9
0.00355
NADPH
-
oxidation of cortisol
0.00372
NADPH
-
temperature not specified in the publication, pH 9
additional information
additional information
-
high- and low Km forms, suggesting 2 isoenzymes, high-Km form represents 90% of total activity in untreated microsomes
-
additional information
additional information
-
substrate specificity and kinetics
-
additional information
additional information
-
substrate specificity and kinetics
-
additional information
additional information
-
the glucocorticoid and xenobiotic reductase activities show cooperative kinetics
-