1.1.1.103: L-threonine 3-dehydrogenase

This is an abbreviated version!
For detailed information about L-threonine 3-dehydrogenase, go to the full flat file.

Reaction

Synonyms

CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.103 L-threonine 3-dehydrogenase

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Results	in table
3	Activating Compound
11521	AA Sequence
2	Application
1	CAS Registry Number
20	Cloned(Commentary)
49	Cofactor
9	Crystallization (Commentary)
4	Disease/ Diagnostics
3	Expression
13	General Information
1	General Stability
75	Inhibitors
32	kcat/KM [mM/s]
4	Ki Value [mM]
97	KM Value [mM]
2	Localization
50	Metals/Ions
40	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
108	Organism
7	Pathway
82	PDB ID
24	pH Optimum
6	pH Range
4	pH Stability
3	pI Value
1	Posttranslational Modification
40	Protein Variants
30	Purification (Commentary)
9	Reaction
1	Reaction Type
67	Reference
37	Source Tissue
36	Specific Activity [micromol/min/mg]
5	Storage Stability
129	Substrates and Products (Substrate)
27	Subunits
53	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
3	Temperature Range [°C]
13	Temperature Stability [°C]
37	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.103 - L-threonine 3-dehydrogenase

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5 entries

acid precipitation, ammonium sulfate, Sephadex G-100, Sephadex G-200

Capra hircus

285715

ammonium sulfate, DEAE-cellulose, 7fold purification

Lithobates catesbeianus

285721

by heat treatment (about 80% pure recombinant TDH protein) and gel filtration

Thermococcus kodakarensis KOD1

698673

by sonication, centrifugation and on a Ni-NTA resin column

Streptomyces sp. 139

698526

by sonication, centrifugation, heating of the soluble fraction, anion-exchange and hydrophobic interaction chromatography

Thermococcus kodakarensis

Q5JI69

695358

by sonication, heating of the cell lysate, anion exchange and hydrophobic interaction chromatography

Thermococcus kodakarensis

Q5JI69

699854

chromatography on Q-Sepharose and Reactive Green 19-agarose

Acetoanaerobium sticklandii

E3PS87

729112

DEAE Sepharose FF, Affi-Gel blue, Sephacryl S-200, Matrix Gel red A, Matrix gel Green A

Sus scrofa

285705

DEAE-cellulose, ammonium sulfate, DEAE-cellulose, Blue dextran-Sepharose, Sephadex G-200, amylamine sepharose, Sephadex G-200

Gallus gallus

285717

DEAE-cellulose, hydrophobic interaction chromatography, Affi-Gel Blue, Matrex Red A

hydroxyapatite

MnCl2, ammonium sulfate, calcium phosphate gel, protamine sulfate, DEAE-cellulose, 7fold purification

Staphylococcus aureus

285720

Ni-chelating column chromatography and Superdex 200 gel filtration

Mus musculus

Q8K3F7

738933

Ni-NTA agarose column chromatography

Escherichia coli

P07913

738860

Ni2+-Sepharose column chromatography and Superdex 75 gel filtration

Cupriavidus necator

738606

protamine sulfate, Sephadex G-200, DEAE-cellulose

Arthrobacter sp.

285719

recombinant enzyme 48fold from Escherichia coli to homogeneity by heat treatment and anion exchange chromatography

Pyrococcus furiosus

668567

recombinant enzyme from Escherichia coli

Pyrococcus horikoshii

667071

recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C for 30 min, centrifugation, ion exchange chromatography, and gel filtration

Pyrococcus horikoshii

669546

recombinant His-tagged enzyme 4.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, native enzyme 75.4fold by anion exchange and hydrophobic interaction chromatography, dialysis, hydroxyapatite chromatography, followed by gel filtration

Cupriavidus necator

E5RQ20

721272

recombinant His-tagged enzyme from Escherichia coli strain TOP10 by nickel affinity chromatography

Pyrococcus horikoshii

668536

recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment at 85°C for 30 min, anion exchange and hydrophobic interaction chromatography

Pyrococcus horikoshii

O58389

687461

treatment of cell extract at 60°C, DEAE-Sephadex, Mn2+ activation, Blue 2-Sepharose CL-6B, C38D mutant was purified without Mn2+ activation

Escherichia coli K-12

285702