1.1.1.103: L-threonine 3-dehydrogenase
This is an abbreviated version!
For detailed information about L-threonine 3-dehydrogenase, go to the full flat file.
Reaction
Synonyms
CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase
ECTree
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Inhibitors
Inhibitors on EC 1.1.1.103 - L-threonine 3-dehydrogenase
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1,10-phenanthroline
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1.26 mM, 41% inhibition after 1 h, 82% inhibition after 2 h, no change in remaining activity after removal of 1,10-phenanthroline
5,5'-dithiobis-(2-nitrobenzoic acid)
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0.25 mM, 90% inhibition, 67% activity is recovered after incubation with 1 mM, 2-mercaptoethanol or dithieothreitol for 15 min
dipicolinic acid
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40 mM, 99% inhibition after 1 h, complete loss of enzyme-bound Zn2+
L-2-amino-3-oxobutyrate
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competitive product inhibition by the unstable L-2-amino-3-oxobutyrate only in presence of NADH, which stabilizes
methyl p-nitrobenzenesulfonate
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2 mM, 40% inhibition within 80 min, 65% protection with 250 mM L-threonine, 64% with 250 mM L-threonine methyl ester, 58% with 250 mM L-threonine amide
methylglyoxal
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1.0 mM, 42% inhibition, 2 mM, 63% inhibition, methylglyoxal binds at an allosteric site of the enzyme
methylmethanethiosulfonate
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0.4 mM, 350fold molar excess over enzyme sulfhydryl groups leads to complete inactivation
thionitrobenzoate
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40fold molar excess, gradual 99% loss of enzyme activity
adenosine-5'-diphosphoribose
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competive inhibition vs. NAD+, noncompetitive inhibition vs. L-threonine
EDTA
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50 mM, 99% inhibition after 1 h, complete loss of enzyme-bound Zn2+
EDTA
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retains 65% of its original activity after dialysis at 48°C against a buffer containing 1 mM EDTA. Activity is lost when TDH is heated at 60°C for 40 min and in boiling water for 5 min in the presence of 1 mM EDTA
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iodoacetate reacts with 1 sulfhydryl group per subunit of the enzyme, enzyme retains 15% of its initial activity
iodoacetate
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15% protection against inhibition with 5 mM NAD+, 30% with 5 mM L-threonine, 60-70% protection in the presence of both NAD+ and L-threonine, inactivation occurs more rapidly in the presence of Cd2+; enzyme contains 6 half-cystine residues per subunit, 2 disulfide bonds and 4 sulfhydryl groups
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preincubation with 1 mM, 47% inhibition of L-threonine amide oxidation, 73% inhibition of L-threonine methyl ester oxidation, 59% inhibition of L-serine oxidation, 48% inhibition of D,L-threo-beta-phenylserine oxidation
p-mercuribenzoate
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0.0013 mM, 75% inhibition, completely reversed within 20 min by addition of 0.02-0.2 mM 2-mercaptoethanol or dithiothreitol
no inhibition by Ni2+, Ca2+, Mg2+, Mn2+
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additional information
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no inhibition by Ni2+, Ca2+, Mg2+, Mn2+
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additional information
poor inhibition by K[Fe(CN)6], no inhibition by ethylene diamine tetraacetic acid and ethylene glycol tetraacetic acid, and by trypsin inhibitor T-9378
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additional information
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poor inhibition by K[Fe(CN)6], no inhibition by ethylene diamine tetraacetic acid and ethylene glycol tetraacetic acid, and by trypsin inhibitor T-9378
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additional information
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L-ThrDH is unaffected by EDTA, Li2SO4, MgCl2, MnCl2, CaCl2, NiCl2, CoCl2, BaCl2, HgCl2, CdSO4, CuSO4, ZnCl2, or iodoacetic acid, each at 1 mM
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